| Bacillus halotolerans |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Bacillota (Firmicutes), Class Bacilli, Order Caryophanales, Family Bacillaceae, Genus Bacillus, Bacillus halotolerans
(Delaporte and Sasson 1967) Tindall 2017.
Synonyms: Brevibacterium halotolerans Delaporte and Sasson 1967, Bacillus axarquiensis Ruiz-García et al. 2005, Bacillus
malacitensis Ruiz-García et al. 2005.
(Delaporte and Sasson 1967) Tindall 2017.
Synonyms: Brevibacterium halotolerans Delaporte and Sasson 1967, Bacillus axarquiensis Ruiz-García et al. 2005, Bacillus
malacitensis Ruiz-García et al. 2005.
Gram-positive, round-ended rods, 0.5 x 2.0 µm, occurring singly or in pairs, rarely in
short chains or filaments. Motile by peritrichous flagella. The endospores are mainly
ellipsoidal and subterminally positioned in non-swollen sporangia. No parasporal
crystals. No poly-beta-hydroxybutyrate (PHB) accumulation.
short chains or filaments. Motile by peritrichous flagella. The endospores are mainly
ellipsoidal and subterminally positioned in non-swollen sporangia. No parasporal
crystals. No poly-beta-hydroxybutyrate (PHB) accumulation.
Colonies on TSA are cream-coloured, slightly irregular in shape and bulge upward.
When the medium is supplemented with salt the colonies become mucous. In liquid
medium a thin film is formed at the surface whilst the rest of the medium is uniformly
cloudy. Grows at 15-45 ºC (optimum at 32 ºC), at pH 5-10 (optimum pH 7.2) and in
0-12% (w/v) NaCl (optimum 0.5% NaCl). Haemolysis is produced. Grows in the
presence of lysozyme (0.001%) and in media without yeast extract. No growth on
MacConkey agar. Aerobic.
When the medium is supplemented with salt the colonies become mucous. In liquid
medium a thin film is formed at the surface whilst the rest of the medium is uniformly
cloudy. Grows at 15-45 ºC (optimum at 32 ºC), at pH 5-10 (optimum pH 7.2) and in
0-12% (w/v) NaCl (optimum 0.5% NaCl). Haemolysis is produced. Grows in the
presence of lysozyme (0.001%) and in media without yeast extract. No growth on
MacConkey agar. Aerobic.
Isolated from brackish sediments taken from the mouth of the river Velez in the province of Malaga in southern Spain.
Susceptible to amoxicillin, amoxicillin+clavulanic acid, cephalothin, chloramphenicol, doxycycline, erythromycin, kanamycin, nalidixic
acid, nitrofurantoin, norfloxacin, novobiocin, rifampicin, trimethoprim+sulfametoxazol and vancomycin. Variable for ceftazidime and
colistin. Resistant to aztreonan.
Susceptible to amoxicillin, amoxicillin+clavulanic acid, cephalothin, chloramphenicol, doxycycline, erythromycin, kanamycin, nalidixic
acid, nitrofurantoin, norfloxacin, novobiocin, rifampicin, trimethoprim+sulfametoxazol and vancomycin. Variable for ceftazidime and
colistin. Resistant to aztreonan.
Undetermined.
- Ruiz-Garcia C, Quesada E, Martinez-Checa F, Llamas I, Urdaci MC, Bejar V. Bacillus axarquiensis sp. nov. and Bacillus malacitensis
sp. nov., isolated from river-mouth sediments in southern Spain. Int J Syst Evol Microbiol 2005; 55:1279-1285. - Tindall BJ. The consequences of Bacillus axarquiensis Ruiz-Garcia et al. 2005, Bacillus malacitensis Ruiz-Garcia et al. 2005 and
Brevibacterium halotolerans Delaporte and Sasson 1967 (Approved Lists 1980) being treated as heterotypic synonyms. Int J Syst
Evol Microbiol 2017; 67:175-176. - Ben-Gad D, Gerchman Y. Reclassification of Brevibacterium halotolerans DSM8802 as Bacillus halotolerans comb. nov. Based on
Microbial and Biochemical Characterization and Multiple Gene Sequence. Curr Microbiol 2017; 74:1-5.
Positive results for caseinase, catalase, DNase, gelatinase, beta-galactosidase (ONPG), H2S production (positive from cysteine,
negative from sodium thiosulphate), lecithinase, nitrate reduction, starch hydrolysis, Tween 20 and 80 hydrolysis, Voges-Proskauer
test, acid production from: L-arabinose, arbutin, aesculin, cellobiose, fructose, glucose, glycerol, glycogen, inositol, maltose,
mannose, mannitol, ribose, starch, sorbitol, salicin, sucrose, trehalose, xylitol and D-xylose.
Can utilize: glucose, carboxymethyl cellulose, and cellulose.
Negative results for indole production, methyl red test, oxidase, phenylalanine deaminase, tryptophan, tyrosine hydrolysis, urease
acid production from: D-arabinose, D- and L-arabitol, adonitol, dulcitol, erythritol, D- and L-fucose, galactose, gluconate, inulin, 2- and
5-ketogluconate, lactose, D-lyxose, melezitose, melibiose, methyl alpha-D-mannoside, N-acetylglucosamine, rhamnose, sorbose,
D-turanose, D-tagatose, methyl beta-D-xyloside and L-xylose.
Variable results for arginine dihydrolase, citrate utilization, dihydroxyacetone production, lysine decarboxylase, ornithine
decarboxylase, acid production from: amygdalin, gentiobiose, methyl alpha-D-glucoside and raffinose.
negative from sodium thiosulphate), lecithinase, nitrate reduction, starch hydrolysis, Tween 20 and 80 hydrolysis, Voges-Proskauer
test, acid production from: L-arabinose, arbutin, aesculin, cellobiose, fructose, glucose, glycerol, glycogen, inositol, maltose,
mannose, mannitol, ribose, starch, sorbitol, salicin, sucrose, trehalose, xylitol and D-xylose.
Can utilize: glucose, carboxymethyl cellulose, and cellulose.
Negative results for indole production, methyl red test, oxidase, phenylalanine deaminase, tryptophan, tyrosine hydrolysis, urease
acid production from: D-arabinose, D- and L-arabitol, adonitol, dulcitol, erythritol, D- and L-fucose, galactose, gluconate, inulin, 2- and
5-ketogluconate, lactose, D-lyxose, melezitose, melibiose, methyl alpha-D-mannoside, N-acetylglucosamine, rhamnose, sorbose,
D-turanose, D-tagatose, methyl beta-D-xyloside and L-xylose.
Variable results for arginine dihydrolase, citrate utilization, dihydroxyacetone production, lysine decarboxylase, ornithine
decarboxylase, acid production from: amygdalin, gentiobiose, methyl alpha-D-glucoside and raffinose.
(c) Costin Stoica
| Antibiogram |
| Encyclopedia |
| Culture media |
| Biochemical tests |
| Stainings |
| Images |
| Movies |
| Articles |
| Identification |
| Software |
| R E G N U M PROKARYOTAE |
| Back |