| Geobacillus thermoleovorans |
Taxonomy
Morphology
Growth conditions
Biochemical characters
Ecology
Pathogenicity
References
Phylum Bacillota (Firmicutes), Class Bacilli, Order Caryophanales, Family Bacillaceae, Genus Geobacillus, Geobacillus
thermoleovorans (Zarilla and Perry 1988) Nazina et al. 2001.
Old synonym: Bacillus thermoleovorans Zarilla and Perry 1988.
thermoleovorans (Zarilla and Perry 1988) Nazina et al. 2001.
Old synonym: Bacillus thermoleovorans Zarilla and Perry 1988.
Generally Gram-negative rods, 0.7-1.5 x 2-6 µm. Some strains are motile by
preitrichous flagella. Spores are terminally or subterminally located, slightly swelling
the sporangium.
preitrichous flagella. Spores are terminally or subterminally located, slightly swelling
the sporangium.
Colonies are circular, creamy white in colour, with smooth and glossy or rough
surfaces, entire or irregular margins, 0.5-5 mm in diameter, following incubation at 60
ºC for 18-24 h. Grows between 37-40 ºC and 65-70 ºC, with optimum growth at 60 ºC;
no growth at 30 or 80 ºC. Grows sparsely at pH 5 and 9; optimum growth at pH 6-7.
Tolerates 0.5-1% NaCl. Aerobic; growth is weak in the absence of oxygen.
surfaces, entire or irregular margins, 0.5-5 mm in diameter, following incubation at 60
ºC for 18-24 h. Grows between 37-40 ºC and 65-70 ºC, with optimum growth at 60 ºC;
no growth at 30 or 80 ºC. Grows sparsely at pH 5 and 9; optimum growth at pH 6-7.
Tolerates 0.5-1% NaCl. Aerobic; growth is weak in the absence of oxygen.
Isolated from pasteurized milk, geothermally heated pools and springs and associated soil, marine sediment of a hydrothermal vent
and crude oil.
and crude oil.
Unknown (none).
- Zarilla K, Perry JJ. Bacillus thermoleovorans, sp. nov., a species of obligately thermophilic hydrocarbon utilizing endospore-forming
bacteria. Syst. Appl. Microbiol. 1987; 9:258-264. - Dinsdale AE, Halket G, Coorevits A, Van Landschoot A, Busse HJ, De Vos P, Logan NA. Emended descriptions of Geobacillus
thermoleovorans and Geobacillus thermocatenulatus. Int J Syst Evol Microbiol 2011; 61:1802-1810.
Positive results for catalase, aesculin hydrolysis, gelatinase, nitrate reduction, oxidase, starch hydrolysis, acid production from:
D-fructose, D-glucose, glycerol, maltose, D-mannose, ribose and salicin (weakly).
Can utilize n-alkanes as growth substrate.
Can assimilate: cellobiose, D-fructose, D-galactose, gentiobiose, D-glucose, lactose, maltitol, maltose, maltotriose, mannitol,
D-mannose, melezitose, melibiose, 1-O-methyl beta-D-glucopyranoside, palatinose, D-ribose, sucrose, trehalose, turanose,
N-acetylglucosamine, fumarate, D-gluconate, DL-glycerate, DL-beta-hydroxybutyrate (3-hydroxybutyrate), L-malate, succinate, (weak
when positive), DL-lactate, D-malate (weak when positive), phenylacetate, quinate, D-glucosamine, L-glutamate and L-histidine.
Negative results for arginine dihydrolase, citrate utilization, H2S production, indole production, lysine decarboxylase, ornithine
decarboxylase, tryptophan deaminase, urease, acid production from: adonitol, amygdalin, D- or L-arabinose, D- or L-arabitol, arbutin,
dulcitol, erythritol, D- or L-fucose, gentiobiose, gluconate, inulin, 2- or 5-ketogluconate, D-lyxose, methyl D-mannoside, methyl
xyloside, rhamnose, sorbitol, L-sorbose, D-tagatose, turanose, xylitol or L-xylose.
No assimilation of: adonitol, L-arabinose, D- and L-arabitol, dulcitol, erythritol, L-fucose, L-rhamnose, D-sorbitol, L-sorbose,
D-tagatose, xylitol, cis- and trans-aconitate, 4-aminobutyrate, 4-aminovalerate, benzoate, caprate, caprylate, citrate, m-coumarate,
D-galacturonate, gentisate, D-glucuronate, glutarate, 3- and 4-hydroxybenzoate, itaconate, alpha-ketoglutarate, 5-ketogluconate,
malonate, mucate, 3-phenylpropionate, propionate, protocatechuate, D-saccharate, D-, L- andmeso-tartrate, tricarballylate,
L-aspartate, betaine, ethanolamine, putrescine, trigonelline, tryptamine, L-tryptophan and L-tyrosine.
Variable results for caseinase, ONPG, Voges-Proskauer test, acid production from: cellobiose, galactose, glycogen, mannitol,
melezitose (weak when positive), melibiose, myo-inositol, lactose (weak when positive), methyl D-glucoside, N-acetylglucosamine,
raffinose, starch, sucrose, trehalose and D-xylose.
Variable assimilation of: 2-ketogluconate, aesculin, glycerol, myo-inositol, lactulose, D-lyxose, 1-O- and 3-O-methyl
alpha-D-glucopyranoside, 1-O-methyl alpha-galactopyranoside (weak when positive), 1-O-methyl beta-glucopyranoside, raffinose,
D-xylose, D- and L-alanine, histamine (weak when positive), L-proline (weak when positive) and L-serine.
D-fructose, D-glucose, glycerol, maltose, D-mannose, ribose and salicin (weakly).
Can utilize n-alkanes as growth substrate.
Can assimilate: cellobiose, D-fructose, D-galactose, gentiobiose, D-glucose, lactose, maltitol, maltose, maltotriose, mannitol,
D-mannose, melezitose, melibiose, 1-O-methyl beta-D-glucopyranoside, palatinose, D-ribose, sucrose, trehalose, turanose,
N-acetylglucosamine, fumarate, D-gluconate, DL-glycerate, DL-beta-hydroxybutyrate (3-hydroxybutyrate), L-malate, succinate, (weak
when positive), DL-lactate, D-malate (weak when positive), phenylacetate, quinate, D-glucosamine, L-glutamate and L-histidine.
Negative results for arginine dihydrolase, citrate utilization, H2S production, indole production, lysine decarboxylase, ornithine
decarboxylase, tryptophan deaminase, urease, acid production from: adonitol, amygdalin, D- or L-arabinose, D- or L-arabitol, arbutin,
dulcitol, erythritol, D- or L-fucose, gentiobiose, gluconate, inulin, 2- or 5-ketogluconate, D-lyxose, methyl D-mannoside, methyl
xyloside, rhamnose, sorbitol, L-sorbose, D-tagatose, turanose, xylitol or L-xylose.
No assimilation of: adonitol, L-arabinose, D- and L-arabitol, dulcitol, erythritol, L-fucose, L-rhamnose, D-sorbitol, L-sorbose,
D-tagatose, xylitol, cis- and trans-aconitate, 4-aminobutyrate, 4-aminovalerate, benzoate, caprate, caprylate, citrate, m-coumarate,
D-galacturonate, gentisate, D-glucuronate, glutarate, 3- and 4-hydroxybenzoate, itaconate, alpha-ketoglutarate, 5-ketogluconate,
malonate, mucate, 3-phenylpropionate, propionate, protocatechuate, D-saccharate, D-, L- andmeso-tartrate, tricarballylate,
L-aspartate, betaine, ethanolamine, putrescine, trigonelline, tryptamine, L-tryptophan and L-tyrosine.
Variable results for caseinase, ONPG, Voges-Proskauer test, acid production from: cellobiose, galactose, glycogen, mannitol,
melezitose (weak when positive), melibiose, myo-inositol, lactose (weak when positive), methyl D-glucoside, N-acetylglucosamine,
raffinose, starch, sucrose, trehalose and D-xylose.
Variable assimilation of: 2-ketogluconate, aesculin, glycerol, myo-inositol, lactulose, D-lyxose, 1-O- and 3-O-methyl
alpha-D-glucopyranoside, 1-O-methyl alpha-galactopyranoside (weak when positive), 1-O-methyl beta-glucopyranoside, raffinose,
D-xylose, D- and L-alanine, histamine (weak when positive), L-proline (weak when positive) and L-serine.
(c) Costin Stoica
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