Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Actinomycetota, Class Actinomycetes, Order Micrococcales, Family Micrococcaceae, Genus Kocuria, Kocuria arsenatis
Roman-Ponce et al. 2016.
Species description is based on a single isolate.
Gram-positive cocci, 0.2–0.6 μm, occurring in pairs, tetrads and packets. Endospores
are not formed. Nonmotile.
Colonies are 1.5-2.5 mm in diameter, opaque, smooth and circular with regular
edges and yellow pigmentation after cultivation for 48 h on TSA at 28 ºC. Pink–brown
diffusible pigment is observed after incubation for 3 days in TSB at 28 ºC. In LB, the
temperature range for growth is 20-44 ºC and optimum growth is observed at 28-37
ºC. NaCl is not required for growth, but is tolerated up to 10% (w/v), optimum in 1-3%
NaCl. The pH range for growth 4.0-11.0, optimum at pH 6.0. Grows well in TSA, LB,
NA, ISP 1, ISP 5 and Czapek’s agar.
Isolated from from lateral root tissue of Prosopis laegivata grown on a mine tailing with high arsenic concentration at Villa de la Paz,
San Luis Potosi, Mexico.
Resistant to 20 mM arsenate and arsenite in the mineral medium.
Undetermined.
- Roman-Ponce B., Wang, D., Vasquez-Murrieta, M. S., Chen, W. F., Estrada-De Los Santos, P., Sui, X. H. and Wang, E. T. 2016.
Kocuria arsenatis sp. nov., an arsenic-resistant endophytic actinobacterium associated with Prosopis laegivata grown on high-
arsenic-polluted mine tailing. Int. J. Syst. Evol. Microbiol., 66, 1027-1033.
Positive results for catalase, nitrite reduction and urease.
Can utilize citrate, dextrin, glycogen, Tween 40, Tween 80, L-arabinose, D-fructose, D-gluconic acid, alpha-D-glucose, maltose,
maltrotiose, melezitose, melibiose, D-picose, ribose, sucrose, D-tagatose, trehalose, turanose, D-xylose, acetic acid,
alpha-hydroxybutyric acid, beta-hydroxybutyric, gamma-hydroxyphenylacetic acid, alpha-ketoglutaric acid, alpha-ketovaleric, lactamide,
D-lactic acid methyl ester, L-lactic acid, L-malic acid, L-pyruvic acid methyl ester, succinic acid monomethyl ester, propionic acid,
pyruvic acid, succinic acid, succinamic acid, N-acetylL-glutamic acid, L-alaninamide, D- and L-alanine, L-alanyl glycine, L-asparagine,
L-glutamic acid, L-pyroglutamic acid, L-serine, glycerol, adenosine, 2'-deoxyadenosine, inosine, thymidine, uridine,
adenosine-5'-monophosphate, thymidine-5'-monophosphate, uridine-5'-monophosphate, D-fructose 6-phosphate, D-glucose
6-phosphate and DL-aglycerol phosphate.
Weak utilization of alpha- and beta-cyclodextrin, amygdalin, beta-methyl D-glucoside, beta-methyl D-glucose, palatinose,
L-rhamnose, salicin, xylitol, adenosine-5'-monophosphate and alpha-D-glucose 1-phosphate.
Negative results for strach hydrolysis.
(c) Costin Stoica