| Pseudomonas bubulae |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas bubulae Lick et al. 2020.
Species description is based on a single isolate.
Genus Pseudomonas, Pseudomonas bubulae Lick et al. 2020.
Species description is based on a single isolate.
Gram-negative, straight rods, 0.7 x 1.9 µm, occurring singly or in pairs. Motile.
Non-sporogenous.
Non-sporogenous.
Colonies are circular, convex, ivorycoloured, smooth, round and glossy , 0.5-1.0mm
in diameter, after incubation at 25 ºC for 24h on Standard-I agar or CASO agar with
glucose. Non-fluorescent on King B agar. Growth occurs at 2-33 ºC, optimally at 25-29
ºC. Growth occurs at pH 5.5-8.5 and in the presence of up to 6% (w/v) NaCl.
Non-haemolytic. Strictly aerobic.
in diameter, after incubation at 25 ºC for 24h on Standard-I agar or CASO agar with
glucose. Non-fluorescent on King B agar. Growth occurs at 2-33 ºC, optimally at 25-29
ºC. Growth occurs at pH 5.5-8.5 and in the presence of up to 6% (w/v) NaCl.
Non-haemolytic. Strictly aerobic.
Isolated from refrigerated raw beef.
Undetermined.
- Lick S, Krockel L, Wibberg D, Winkler A, Blom J, Goesmann A, Kalinowski J. Pseudomonas bubulae sp. nov., isolated from beef. Int
J Syst Evol Microbiol 2020; 70:292-301.
Positive results for arginine dihydrolase, acid phosphatase, leucine arylamidase, naphthol-AS-BI-phosphohydrolase, catalase, oxidase.
Can utilize (API 20 NE): D-glucose, L-arabinose, D-mannose, D-mannitol, gluconate, capric acid, malic acid and tri-sodium citrate.
API 50 CHB/E (not clear if utilization or fermentation - admin note): glycerol, D-glucose, D- and L-arabinose, D-fructose, D-ribose,
D-xylose, D-galactose, D-mannose, D- and L-fucose.
Biolog GN2: D-fructose, D-mannitol, D-mannose, pyruvic acid methyl ester, D-glucuronic acid, hydroxy-L-proline, D-serine, urocanic
acid, glycerol, alpha-D-glucose, acetic acid, cis-aconitic acid, citric acid, D-gluconic acid, DL-lactic acid, propionic acid and succinic acid.
Negative results for alkaline phosphatase, cystine arylamidase, aesculin hydrolysis, esterase (C4), esterase lipase (C8), lipase (C14),
alpha-fucosidase, alpha- and beta-galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, gelatin hydrolysis, indole
production, alphs-mannosidase, nitrate reduction, N-acetyl-beta-glucosaminidase, trypsin, alpha-chymotrypsin, urease, valine
arylamidase and acid production from glucose.
Negative API 20 NE: adipic acid N-acetyl glucosamine, maltose, phenylacetate.
Negative API 50 CHB/E: erythritol, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, L-sorbose, dulcitol, inositol, xylitol, D-lyxose,
D-arabitol, D-sorbitol, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine, amygdalin, arbutin,
aesculin ferric citrate, salicin, inulin, melezitose, raffinose, starch, glycogen, gentiobiose, turanose, D-tagatose, L-arabitol, gluconate, 2-
and 5-ketogluconate.
Negative Biolog GN2: alpha-cyclodextrin, dextrin, glycogen, Tween 40 and 80, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine,
adonitol, L-arabinose, D-arabitol, cellobiose, D-galactose, gentiobiose, lactose, lactulose, maltose, melibiose, methyl
beta-D-glucoside, D-psicose, raffinose, L-rhamnose, turanose, xylitol, succinic acid mono-methyl ester, formic acid, D-galactonic acid
lactone, D-galacturonic acid, D-glucosaminic acid, alpha-hydroxybutyric acid, p-hydroxyphenylacetic acid, alpha-ketobutyric acid,
alpha-ketovaleric acid, malonic acid, sebacic acid, glucuronamide, L-alaninamide, glycyl L-aspartic acid, L-phenylalanine, L-threonine,
DL-carnitine, thymidine, phenylethylamine, 2-aminoethanol, 2,3-butanediol, DL-alpha-glycerol-phosphate, alpha-D-glucose
1-phosphate, D-glucose 6-phosphate, myo-inositol, D-sorbitol, sucrose, gamma-hydroxybutyric acid, itaconic acid, succinamic acid,
L-alanyl glycine, L-aspartic acid, glycyl L-glutamic acid, L-leucine, L-ornithine, L-serine, uridine and putrescine.
Variable API 50 CHB/E: D-fructose, D-mannitol, cellobiose, maltose, lactose, melibiose, sucrose and trehalose.
Variable Biolog GN2: i-erythritol, trehalose, beta-hydroxybutyric acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid, D- and
L-alanine, L-asparagine, L-histidine, L-proline, L-pyroglutamic acid and gamma-aminobutyric acid.
Can utilize (API 20 NE): D-glucose, L-arabinose, D-mannose, D-mannitol, gluconate, capric acid, malic acid and tri-sodium citrate.
API 50 CHB/E (not clear if utilization or fermentation - admin note): glycerol, D-glucose, D- and L-arabinose, D-fructose, D-ribose,
D-xylose, D-galactose, D-mannose, D- and L-fucose.
Biolog GN2: D-fructose, D-mannitol, D-mannose, pyruvic acid methyl ester, D-glucuronic acid, hydroxy-L-proline, D-serine, urocanic
acid, glycerol, alpha-D-glucose, acetic acid, cis-aconitic acid, citric acid, D-gluconic acid, DL-lactic acid, propionic acid and succinic acid.
Negative results for alkaline phosphatase, cystine arylamidase, aesculin hydrolysis, esterase (C4), esterase lipase (C8), lipase (C14),
alpha-fucosidase, alpha- and beta-galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, gelatin hydrolysis, indole
production, alphs-mannosidase, nitrate reduction, N-acetyl-beta-glucosaminidase, trypsin, alpha-chymotrypsin, urease, valine
arylamidase and acid production from glucose.
Negative API 20 NE: adipic acid N-acetyl glucosamine, maltose, phenylacetate.
Negative API 50 CHB/E: erythritol, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, L-sorbose, dulcitol, inositol, xylitol, D-lyxose,
D-arabitol, D-sorbitol, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine, amygdalin, arbutin,
aesculin ferric citrate, salicin, inulin, melezitose, raffinose, starch, glycogen, gentiobiose, turanose, D-tagatose, L-arabitol, gluconate, 2-
and 5-ketogluconate.
Negative Biolog GN2: alpha-cyclodextrin, dextrin, glycogen, Tween 40 and 80, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine,
adonitol, L-arabinose, D-arabitol, cellobiose, D-galactose, gentiobiose, lactose, lactulose, maltose, melibiose, methyl
beta-D-glucoside, D-psicose, raffinose, L-rhamnose, turanose, xylitol, succinic acid mono-methyl ester, formic acid, D-galactonic acid
lactone, D-galacturonic acid, D-glucosaminic acid, alpha-hydroxybutyric acid, p-hydroxyphenylacetic acid, alpha-ketobutyric acid,
alpha-ketovaleric acid, malonic acid, sebacic acid, glucuronamide, L-alaninamide, glycyl L-aspartic acid, L-phenylalanine, L-threonine,
DL-carnitine, thymidine, phenylethylamine, 2-aminoethanol, 2,3-butanediol, DL-alpha-glycerol-phosphate, alpha-D-glucose
1-phosphate, D-glucose 6-phosphate, myo-inositol, D-sorbitol, sucrose, gamma-hydroxybutyric acid, itaconic acid, succinamic acid,
L-alanyl glycine, L-aspartic acid, glycyl L-glutamic acid, L-leucine, L-ornithine, L-serine, uridine and putrescine.
Variable API 50 CHB/E: D-fructose, D-mannitol, cellobiose, maltose, lactose, melibiose, sucrose and trehalose.
Variable Biolog GN2: i-erythritol, trehalose, beta-hydroxybutyric acid, alpha-ketoglutaric acid, quinic acid, bromosuccinic acid, D- and
L-alanine, L-asparagine, L-histidine, L-proline, L-pyroglutamic acid and gamma-aminobutyric acid.
(c) Costin Stoica
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