| Pseudomonas canadensis |
Description is based on API 20 NE (bioMerieux), Biolog PM1 and PM2A Microplate results.
Positive results for arginine dihydrolase, gelatinase (negative in Hofmann's study [3]), oxidase and urease.
Can assimilate (API 20 NE): potassium gluconate, capric acid, adipic acid and trisodium citrate.
Can utilize: i-erythritol, sucrose, trehalose, melibiose, L-rhamnose, L-phenylalanine, D-xylitol, N-acetyl-D-glucosamine, itaconic acid,
putrescine, succinic acid, L-aspartic acid, L-proline, D-mannose, D-gluconic acid, L-lactic acid, D-mannitol, L-glutamic acid, DL-malic
acid, Tween 20, D-fructose, acetic acid, alpha-D-glucose, L-asparagine, alpha-ketoglutaric acid, L-glutamine, adenosine, citric acid,
fumaric acid, bromosuccinic acid, propionic acid, inosine, L-serine, D- and L-alanine, L-alanyl-glycine, monomethylsuccinate,
methylpyruvate, L-malic acid, pyruvic acid, gaminobutyric acid, caproic acid, D-glucosamine, beta-hydroxybutyric acid, malonic acid,
quinic acid, succinamic acid, L-arginine, hydroxy-L-proline, L- isoleucine, L-leucine, L-pyroglutamic acid, L-valine, D,L-carnitine,
glycerol, L-arabinose, D-saccharic acid, D-galactose, D-galactonic acid g-lactone, mucic acid, D-glucosaminic acid and D-arabitol.
Negative results for aesculin hydrolysis, nitrate reduction, indole production, beta-glucosidase, beta-galactosidase, lysine
decarboxylase and ornithine decarboxylase.
No utilization of: lactose, maltose, D-fructose 6-phosphate, alpha-hydroxyglutaric acid g-lactone, glycyl-L-proline, phenylethylamine,
1,2-propanediol, D-glucose 6-phosphate, sebacic acid, Tween 40, Tween 80 and formic acid.
Positive results for arginine dihydrolase, gelatinase (negative in Hofmann's study [3]), oxidase and urease.
Can assimilate (API 20 NE): potassium gluconate, capric acid, adipic acid and trisodium citrate.
Can utilize: i-erythritol, sucrose, trehalose, melibiose, L-rhamnose, L-phenylalanine, D-xylitol, N-acetyl-D-glucosamine, itaconic acid,
putrescine, succinic acid, L-aspartic acid, L-proline, D-mannose, D-gluconic acid, L-lactic acid, D-mannitol, L-glutamic acid, DL-malic
acid, Tween 20, D-fructose, acetic acid, alpha-D-glucose, L-asparagine, alpha-ketoglutaric acid, L-glutamine, adenosine, citric acid,
fumaric acid, bromosuccinic acid, propionic acid, inosine, L-serine, D- and L-alanine, L-alanyl-glycine, monomethylsuccinate,
methylpyruvate, L-malic acid, pyruvic acid, gaminobutyric acid, caproic acid, D-glucosamine, beta-hydroxybutyric acid, malonic acid,
quinic acid, succinamic acid, L-arginine, hydroxy-L-proline, L- isoleucine, L-leucine, L-pyroglutamic acid, L-valine, D,L-carnitine,
glycerol, L-arabinose, D-saccharic acid, D-galactose, D-galactonic acid g-lactone, mucic acid, D-glucosaminic acid and D-arabitol.
Negative results for aesculin hydrolysis, nitrate reduction, indole production, beta-glucosidase, beta-galactosidase, lysine
decarboxylase and ornithine decarboxylase.
No utilization of: lactose, maltose, D-fructose 6-phosphate, alpha-hydroxyglutaric acid g-lactone, glycyl-L-proline, phenylethylamine,
1,2-propanediol, D-glucose 6-phosphate, sebacic acid, Tween 40, Tween 80 and formic acid.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas canadensis Tambong et al. 2017.
Species description is based on a single isolate.
Genus Pseudomonas, Pseudomonas canadensis Tambong et al. 2017.
Species description is based on a single isolate.
Gram-negative, rods, 0.5 x 2.3 µm long. Motile by one or multiple polar flagella.
Non-spore-forming.
Non-spore-forming.
After 48 h incubation on King’s B agar, colonies are white-yellowish and circular, 4
mm in diameter, convex with regular margins and produce fluorescent pigments.
Tolerant to different NaCl concentrations up to 4%, and grows at 4 ºC with optimal
growth at 30 ºC and no growth at 40 ºC. Haemolysis is not produced. Aerobic.
mm in diameter, convex with regular margins and produce fluorescent pigments.
Tolerant to different NaCl concentrations up to 4%, and grows at 4 ºC with optimal
growth at 30 ºC and no growth at 40 ºC. Haemolysis is not produced. Aerobic.
Isolated from soil samples collected from a corn-alfalfa rotation plott under long-term (>40 years) mineral fertilization from Woodslee,
Ontario, Canada.
Exhibited in vitro antagonistic properties against fungal pathogens.
Ontario, Canada.
Exhibited in vitro antagonistic properties against fungal pathogens.
Undetermined.
- Tambong JT, Xu R, Bromfield ESP. Pseudomonas canadensis sp. nov., a biological control agent isolated from a field plot under
long-term mineral fertilization. Int J Syst Evol Microbiol 2017; 67:889-895. - Hofmann K, Huptas C, Doll EV, Scherer S, Wenning M. Pseudomonas haemolytica sp. nov., isolated from raw milk and skimmed
milk concentrate. Int J Syst Evol Microbiol 2020; 70:2339-2347. - Hofmann K, Woller A, Huptas C, Wenning M, Scherer S, Doll EV. Pseudomonas cremoris sp. nov., a novel proteolytic species
isolated from cream. Int J Syst Evol Microbiol 2021; 71:4597.
(c) Costin Stoica
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