Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas crudilactis Schlusselhuber et al. 2022.
Species description is based on a single isolate.
Gram-negative rods. Motile. Non-spore-forming.
Colonies are rounds, convex, mucoid, beige colored, with regular margins (3-5 mm)
on TSA-YE after incubation for 48 h at 28 ºC. No fluorescent pigments or pyocyanin
production. Grows at temperature range of 4-30 ºC (optimum 28 ºC),at pH 5-9
(optimum pH 6.5) and 0-5% NaCl (optimum 0% NaCl).
Isolated from bovine raw milk in France.
Undetermined.
- Schlusselhuber M, Girard L, Cousin FJ, Lood C, De Mot R, Goux D, Desmasures N. Pseudomonas crudilactis sp. nov., isolated from
raw milk in France. Antonie Van Leeuwenhoek 2021; 114:719-730.
Positive results for arginine dihydrolase, catalase, protease, phospholipase, oxidase and urease.
Can assimilate (API 20 NE): D-glucose, L-arabinose, D-mannose, D-mannitol, N-acetylglucosamine, D-maltose, potassium
gluconate, capric acid, malic acid and trisodium citrate.
Can utilize (Biolog GEN III): D-maltose, D-cellobiose, gentiobiose, stachyose, D-raffinose, D-melibiose, D-salicin,
N-acetyl-beta-D-mannosamine, N-acetyl-D-galactosamine, N-acetyl neuraminic acid, alpha-D-glucose, D-mannose, D-fructose,
D-galactose, 3-methyl glucose, D- and L-fucose, inosine, D-sorbitol, D-mannitol, D-arabitol, myo-inositol, glycerol,
D-fructose-6-phosphate, D-aspartic acid, D-serine, glycyl-L-proline, L-alanine, L-arginine, L-aspartic acid, L-glutamic acid,
L-pyroglutamic acid, pectin, D-galacturonic acid, L-galactonic acid lactone, D-gluconic acid, glucuronamide, mucic acid, D-saccharic
acid, methyl pyruvate, D-lactic acid methyl ester, D-malic acid, Tween 40, delta-aminobutyric acid, alpha-hydroxybutyric acid,
acetoacetic acid and formic acid.
Negative results for aesculin hydrolysis, gelatin hydrolysis, beta-galactosidase, indole production, lipase, nitrate reduction, and
D-glucose fermentation.
No assimilation of: D-maltose, adipic acid and phenylacetic acid.
No utilization of: dextrin, D-maltose, D-trehalose, sucrose, D-turanose, alpha-D-lactose, beta-methyl-D-glucoside, L-rhamnose,
D-glucose-6-phosphate, gelatin, L-histidine, L-serine, D-glucuronic acid, quinic acid, p-hydroxy-phenylacetic acid, L-lactic acid, citric
acid, alpha-ketoglutaric acid, L-malic acid, bromo-succinic acid, beta-hydroxy-D,L-butyric acid, alpha-ketobutyric acid, propionic acid
and acetic acid.
(c) Costin Stoica