| Pseudomonas daroniae |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas daroniae Bueno-Gonzalez et al. 2019.
Genus Pseudomonas, Pseudomonas daroniae Bueno-Gonzalez et al. 2019.
Gram-negative, slightly curved rods, 1.7 x 0.4 µm. Motile by a polar flagellum.
Non-spore-forming..
Non-spore-forming..
Colonies on KB agar are cream-coloured, circular and convex, with entire margins
and measure 2 mm in diameter after 48 h of incubation at 25 ºC. One from four
strains produced fluorescent pigment. Growth is optimum at 28 ºC and pH 6.0-8.0,
but strains can grow at 33 ºC and weakly at 37 ºC. Only one strain was able to grow
small colonies on KB at refrigeration temperatures (4-10 ºC) and at pH 9.0. All strains
grow on 3.5% (w/v) NaCl supplemented TSA plates, and only one grows on 5.5%
(w/v) NaCl. Strictly aerobic.
and measure 2 mm in diameter after 48 h of incubation at 25 ºC. One from four
strains produced fluorescent pigment. Growth is optimum at 28 ºC and pH 6.0-8.0,
but strains can grow at 33 ºC and weakly at 37 ºC. Only one strain was able to grow
small colonies on KB at refrigeration temperatures (4-10 ºC) and at pH 9.0. All strains
grow on 3.5% (w/v) NaCl supplemented TSA plates, and only one grows on 5.5%
(w/v) NaCl. Strictly aerobic.
Isolated from oak trees in Southern England.
Isolated from Quercus robur stem tissue displaying symptoms of acute oak decline.
- Bueno-Gonzalez V, Brady C, Denman S, Plummer S, Allainguillaume J, Arnold D. Pseudomonas daroniae sp. nov. and
Pseudomonas dryadis sp. nov., isolated from pedunculate oak affected by acute oak decline in the UK. Int J Syst Evol Microbiol
2019; 69:3368-3376.
Description is based mostly on API 20 NE, API 50 CHB/E and Biolog GN2 MicroPlate results.
Positive results for catalase, oxidase, acid production (API 50 CHB/E) from: glycerol, L-arabinose, galactose, D-glucose, D-fructose,
D-mannose, D-mannitol, D-lyxose (weakly) and D-arabitol (weakly).
Can assimilate (API 20 NE): D-glucose, L-arabinose, D-mannose, D-mannitol, potassium gluconate, caprate, malate and citrate.
Can utilize (Biolog GN2): L-arabinose, D-fructose, D-galactose, alpha-D-glucose, D-mannitol, D-mannose, pyruvic acid methyl ester,
succinic acid mono-methylester, acetic acid, cis-aconitic acid, citric acid, D-galacturonic acid, D-gluconic acid, D-glucuronic acid,
gamma-hydroxybutyric acid, itaconic acid, alpha-ketoglutaric acid, D,L-lactic acid, quinic acid, succinic acid, bromosuccinic acid,
succinamic acid, glucuronamide, L-alaninamide, D- and L-alanine, L-aspargine, L-aspartic acid, L-glutamic acid, hydroxy-L-proline,
L-proline, L-pyroglutamic acid, L-serine, gamma-aminobutyric acid, 2-aminoethanol and glycerol.
Negative results for arginine dihydrolase, beta-glucosidase, beta-galactosidase, gelatinase, indole production, nitrate reduction to
nitrites or N2, urease, acid production from: erythritol, D-arabinose, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, L-sorbose,
L-rhamnose, dulcitol, inositol, D-sorbitol, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine,
amygdalin, arbutin, aesculin ferric citrate, salicin, cellobiose, lactose, inulin, melezitose, raffinose, starch, glycogen, xylitol, turanose,
D-tagatose, L-fucose, L-arabitol, gluconate, 2- and 5-ketogluconate.
No utilization of N-acetyl-glucosamine, maltose, adipate and phenylacetate.
No utilization of: malonic acid, sucrose, trehalose, alpha-cyclodextrin, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, adonitol,
cellobiose, i-erythritol, L-fucose, gentiobiose, m-inositol, lactose, lactulose, maltose, melibiose, raffinose, L-rhamnose, D-sorbitol,
xylitol, D-glucosaminic acid, p-hydroxy-phenylacetic acid, sebacic acid, glycyl-L-aspartic acid, L-histidine, L-leucine, L-ornithine,
L-phenylalanine, D-serine, inosine, uridine, thymidine, phenylethylamine, alpha-D-glucose-1-phosphate and alpha-ketovaleric acid.
Variable results for acid production from D-glucose and utilization of: L-alanil-glycine, urocanic acid, D-arabitol, dextrin, formic acid,
glycogen, D-psicose, turanose, alpha- and beta-hydroxybutyric acid, alpha-ketobutyric acid, alpha-ketovaleric acid, propionic acid,
D-saccharic acid, D,L-carnitine, putrescine, 2,3-butanediol and D,L,alpha-glycerol phosphate.
Positive results for catalase, oxidase, acid production (API 50 CHB/E) from: glycerol, L-arabinose, galactose, D-glucose, D-fructose,
D-mannose, D-mannitol, D-lyxose (weakly) and D-arabitol (weakly).
Can assimilate (API 20 NE): D-glucose, L-arabinose, D-mannose, D-mannitol, potassium gluconate, caprate, malate and citrate.
Can utilize (Biolog GN2): L-arabinose, D-fructose, D-galactose, alpha-D-glucose, D-mannitol, D-mannose, pyruvic acid methyl ester,
succinic acid mono-methylester, acetic acid, cis-aconitic acid, citric acid, D-galacturonic acid, D-gluconic acid, D-glucuronic acid,
gamma-hydroxybutyric acid, itaconic acid, alpha-ketoglutaric acid, D,L-lactic acid, quinic acid, succinic acid, bromosuccinic acid,
succinamic acid, glucuronamide, L-alaninamide, D- and L-alanine, L-aspargine, L-aspartic acid, L-glutamic acid, hydroxy-L-proline,
L-proline, L-pyroglutamic acid, L-serine, gamma-aminobutyric acid, 2-aminoethanol and glycerol.
Negative results for arginine dihydrolase, beta-glucosidase, beta-galactosidase, gelatinase, indole production, nitrate reduction to
nitrites or N2, urease, acid production from: erythritol, D-arabinose, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, L-sorbose,
L-rhamnose, dulcitol, inositol, D-sorbitol, methyl alpha-D-mannopyranoside, methyl alpha-D-glucopyranoside, N-acetylglucosamine,
amygdalin, arbutin, aesculin ferric citrate, salicin, cellobiose, lactose, inulin, melezitose, raffinose, starch, glycogen, xylitol, turanose,
D-tagatose, L-fucose, L-arabitol, gluconate, 2- and 5-ketogluconate.
No utilization of N-acetyl-glucosamine, maltose, adipate and phenylacetate.
No utilization of: malonic acid, sucrose, trehalose, alpha-cyclodextrin, N-acetyl-D-galactosamine, N-acetyl-D-glucosamine, adonitol,
cellobiose, i-erythritol, L-fucose, gentiobiose, m-inositol, lactose, lactulose, maltose, melibiose, raffinose, L-rhamnose, D-sorbitol,
xylitol, D-glucosaminic acid, p-hydroxy-phenylacetic acid, sebacic acid, glycyl-L-aspartic acid, L-histidine, L-leucine, L-ornithine,
L-phenylalanine, D-serine, inosine, uridine, thymidine, phenylethylamine, alpha-D-glucose-1-phosphate and alpha-ketovaleric acid.
Variable results for acid production from D-glucose and utilization of: L-alanil-glycine, urocanic acid, D-arabitol, dextrin, formic acid,
glycogen, D-psicose, turanose, alpha- and beta-hydroxybutyric acid, alpha-ketobutyric acid, alpha-ketovaleric acid, propionic acid,
D-saccharic acid, D,L-carnitine, putrescine, 2,3-butanediol and D,L,alpha-glycerol phosphate.
(c) Costin Stoica
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