| Pseudomonas kielensis |
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Pseudomonas, Pseudomonas kielensis Gieschler et al. 2021.
Genus Pseudomonas, Pseudomonas kielensis Gieschler et al. 2021.
Gram-negative rods, 0.9-1.7 x 2.5-4.7 µm. Motile by one polar flagellum.
Non-spore-forming.
Non-spore-forming.
Colonies are 1.0-2.0mm in diameter, smooth, beige, shining and round after
incubation at 30 ºC for 24h on TSA. Beta-haemolysis is not produced on Columbia
agar supplemented with 5% sheep blood. Grows on R2A agar and under anaerobic
conditions but not on cetrimide agar. Fluorescent pigments are not produced on King
A and B. Grows at temperatures between 4-30 ºC (optimum 20-25 ºC), at pH values
6.0-8.0 (optimum pH 6.5-7.5) and in the presence of 0-4% (w/v) NaCl (optimum
0.5-1.0%).
incubation at 30 ºC for 24h on TSA. Beta-haemolysis is not produced on Columbia
agar supplemented with 5% sheep blood. Grows on R2A agar and under anaerobic
conditions but not on cetrimide agar. Fluorescent pigments are not produced on King
A and B. Grows at temperatures between 4-30 ºC (optimum 20-25 ºC), at pH values
6.0-8.0 (optimum pH 6.5-7.5) and in the presence of 0-4% (w/v) NaCl (optimum
0.5-1.0%).
Isolated from raw cow's milk in Germany.
Undetermined.
- Gieschler S, Fiedler G, Bohnlein C, Grimmler C, Franz CMAP, Kabisch J. Pseudomonas kielensis sp. nov. and Pseudomonas
baltica sp. nov., isolated from raw milk in Germany. Int J Syst Evol Microbiol 2019; 71:0.
Positive results for arginine dihydrolase, catalase, citrate utilization, nitrate reduction, oxidase and Voges-Proskauer reaction.
Can utilize as sole carbon source: glycerin, L-arabinose, D-ribose, D-xylose, D-glucose, D-fructose, D-mannose, inositol and sucrose.
Assimilation of D-tagatose, D-fucose and 5-ketogluconate is weakly positive.
Negative results for beta-galactosidase, gelatin hydrolysis, H2S production, indole production, denitrification, lysine decarboxylase,
ornithine decarboxylase, starch hydrolysis, tryptophan deaminase and urease.
No utilization of: erythritol, D-arabinose, L-xylose, D-adonitol, methyl beta-dxylopyranose, D-galactose, L-sorbose, L-rhamnose,
dulcitol, D-mannitol, D-sorbitol, methyl alpha-D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin,
aesculin, salicin, cellobiose, maltose, lactose, melibiose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol,
gentiobiose, turanose, D-lyxose, L-fucose, D- and L-arabitol, potassium-gluconate and 2-ketogluconate.
Can utilize as sole carbon source: glycerin, L-arabinose, D-ribose, D-xylose, D-glucose, D-fructose, D-mannose, inositol and sucrose.
Assimilation of D-tagatose, D-fucose and 5-ketogluconate is weakly positive.
Negative results for beta-galactosidase, gelatin hydrolysis, H2S production, indole production, denitrification, lysine decarboxylase,
ornithine decarboxylase, starch hydrolysis, tryptophan deaminase and urease.
No utilization of: erythritol, D-arabinose, L-xylose, D-adonitol, methyl beta-dxylopyranose, D-galactose, L-sorbose, L-rhamnose,
dulcitol, D-mannitol, D-sorbitol, methyl alpha-D-mannoside, methyl alpha-D-glucoside, N-acetylglucosamine, amygdalin, arbutin,
aesculin, salicin, cellobiose, maltose, lactose, melibiose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol,
gentiobiose, turanose, D-lyxose, L-fucose, D- and L-arabitol, potassium-gluconate and 2-ketogluconate.
(c) Costin Stoica
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