| Thiopseudomonas alkaliphila |
Description is based mostly on API ZYM and API 50 CH (bioMerieux) results.
Positive results for catalase, leucine arylamidase and oxidase.
Can oxidize L-arabinose, D-fucose, D-galactose, D-glucose, D-mannose, D-ribose and D-xylose.
Negative results for alkaline phosphatase, cystine arylamidase, alpha-chymotrypsin, aesculin hydrolysis, alpha-fucosidase, gelatinase,
alpha- and beta-galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, H2S production, indole production, lipase,
alpha-mannosidase, nitrate or nitrite reduction, naphtol-AS-BI-phosphohydrolase, N-acetyl-beta-glucosaminidase, trypsin, urease and
valine arylamidase.
No utilization of: citrate, glycerol, erythritol, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, D-fructose, L-sorbose, dulcitol, inositol,
D-mannitol, D-sorbitol, methyl alpha-D-mannopyranoside, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose,
lactose, sucrose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose,
L-fucose, D-and L-arabitol, potassium gluconate, potassium 2- and 5-ketogluconate.
Variable results for esterase lipase and acid phosphastase.
Variable for the oxidation of D-arabinose, L-rhamnose and melibiose.
Positive results for catalase, leucine arylamidase and oxidase.
Can oxidize L-arabinose, D-fucose, D-galactose, D-glucose, D-mannose, D-ribose and D-xylose.
Negative results for alkaline phosphatase, cystine arylamidase, alpha-chymotrypsin, aesculin hydrolysis, alpha-fucosidase, gelatinase,
alpha- and beta-galactosidase, alpha- and beta-glucosidase, beta-glucuronidase, H2S production, indole production, lipase,
alpha-mannosidase, nitrate or nitrite reduction, naphtol-AS-BI-phosphohydrolase, N-acetyl-beta-glucosaminidase, trypsin, urease and
valine arylamidase.
No utilization of: citrate, glycerol, erythritol, L-xylose, D-adonitol, methyl beta-D-xylopyranoside, D-fructose, L-sorbose, dulcitol, inositol,
D-mannitol, D-sorbitol, methyl alpha-D-mannopyranoside, N-acetylglucosamine, amygdalin, arbutin, salicin, cellobiose, maltose,
lactose, sucrose, trehalose, inulin, melezitose, raffinose, starch, glycogen, xylitol, gentiobiose, turanose, D-lyxose, D-tagatose,
L-fucose, D-and L-arabitol, potassium gluconate, potassium 2- and 5-ketogluconate.
Variable results for esterase lipase and acid phosphastase.
Variable for the oxidation of D-arabinose, L-rhamnose and melibiose.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Thiopseudomonas, Thiopseudomonas alkaliphila (Drobish et al. 2016) Rudra and Gupta 2021.
Basonym: Oblitimonas alkaliphila Drobish et al. 2016.
Genus Thiopseudomonas, Thiopseudomonas alkaliphila (Drobish et al. 2016) Rudra and Gupta 2021.
Basonym: Oblitimonas alkaliphila Drobish et al. 2016.
Gram-negative, pleomorphic cells, alternating between both cocci and bacillary forms,
depending on the age of the culture. Non-motile.
depending on the age of the culture. Non-motile.
Colonies on HIA with 5% rabbit blood are convex, circular in shape and around 1-2 mm
in diameter. Produce a light brown pigment on heart infusion tyrosine agar. Growth
occurs at 10-42 ºC (optimum, 20-35 ºC). Optimal growth occurs after incubation for 48 h
at 35 ºC in a candle jar. It is able to grow under alkalitolerant conditions; up to pH 11
(optimal is pH 7.5). Growth occurs in the presence of 0-8% (w/v) NaCl (optimum, 4-6%).
Grows on MacConkey agar and Salmonella Shigella agar, but not on Cetrimide agar.
Aerobic, microaerophilic.
in diameter. Produce a light brown pigment on heart infusion tyrosine agar. Growth
occurs at 10-42 ºC (optimum, 20-35 ºC). Optimal growth occurs after incubation for 48 h
at 35 ºC in a candle jar. It is able to grow under alkalitolerant conditions; up to pH 11
(optimal is pH 7.5). Growth occurs in the presence of 0-8% (w/v) NaCl (optimum, 4-6%).
Grows on MacConkey agar and Salmonella Shigella agar, but not on Cetrimide agar.
Aerobic, microaerophilic.
Isolated from human urine (3 strains), leg tissue (2 strains), foot wound, lung tissue and deep liver.
Undetermined. Isolated from human clinical samples.
- Drobish AM, Emery BD, Whitney AM, Lauer AC, Metcalfe MG, McQuiston JR. Oblitimonas alkaliphila gen. nov., sp. nov., in the family
Pseudomonadaceae, recovered from a historical collection of previously unidentified clinical strains. Int J Syst Evol Microbiol
2016; 66:3063-3070. - Rudra B, Gupta RS. Phylogenomic and comparative genomic analyses of species of the family Pseudomonadaceae: Proposals
for the genera Halopseudomonas gen. nov. and Atopomonas gen. nov., merger of the genus Oblitimonas with the genus
Thiopseudomonas, and transfer of some misclassified species of the genus Pseudomonas into other genera. Int J Syst Evol
Microbiol 2021; 71:5011.
(c) Costin Stoica
| Antibiogram |
| Encyclopedia |
| Culture media |
| Biochemical tests |
| Stainings |
| Images |
| Movies |
| Articles |
| Identification |
| Software |
| R E G N U M PROKARYOTAE |
| Back |