| Thiopseudomonas denitrificans |
Description is based mostly on API ZYM and API 20 NE (bioMerieux) results.
Positive results for catalase, esterase (C4), esterase lipase (C8), leucine arylamidase, denitrification, naphthol-ASBI-phosphohydrolase
and oxidase.
Malate is assimilated, sulfide is oxidized anaerobically with nitrate as electron acceptor.
Negative results for acid and alkaline phosphatase, arginine dihydrolase, aesculin hydrolysis, cystine arylamidase, alpha-fucosidase,
gelatinase, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, indole production, lipase (C14),
alpha-mannosidase, N-acetyl-beta-glucosaminidase, trypsin, alpha-chymotrypsin, urease, valine arylamidase, acid production from
glucose.
No assimilation of: sucrose, D-glucose, arabinose, mannose, mannitol, N-acetylglucosamine, maltose, gluconate, caprate, adipate,
citrate and phenylacetate.
Positive results for catalase, esterase (C4), esterase lipase (C8), leucine arylamidase, denitrification, naphthol-ASBI-phosphohydrolase
and oxidase.
Malate is assimilated, sulfide is oxidized anaerobically with nitrate as electron acceptor.
Negative results for acid and alkaline phosphatase, arginine dihydrolase, aesculin hydrolysis, cystine arylamidase, alpha-fucosidase,
gelatinase, alpha- and beta-galactosidase, beta-glucuronidase, alpha- and beta-glucosidase, indole production, lipase (C14),
alpha-mannosidase, N-acetyl-beta-glucosaminidase, trypsin, alpha-chymotrypsin, urease, valine arylamidase, acid production from
glucose.
No assimilation of: sucrose, D-glucose, arabinose, mannose, mannitol, N-acetylglucosamine, maltose, gluconate, caprate, adipate,
citrate and phenylacetate.
Taxonomy
Morphology
Cultural characteristics
Biochemical characters
Ecology
Pathogenicity
References
Phylum Pseudomonadota (Proteobacteria), Class Gammaproteobacteria, Order Pseudomonadales, Family Pseudomonadaceae,
Genus Thiopseudomonas, Thiopseudomonas denitrificans Tan et al. 2015, type species of the genus.
Genus Thiopseudomonas, Thiopseudomonas denitrificans Tan et al. 2015, type species of the genus.
Gram-negative rods, 0.4-0.7 x 1.0-1.5 µm. Motile by means of a polar flagellum.
Colonies are translucent at the edge and dark yellow in the centre after 3 days
incubation at 30 ºC on TSA plates. Growth occurs at 10-37 ºC (optimum, 30 ºC), at pH
6.0-10.0 (optimum, pH 8.0) and in the presence of 0-3% (w/v) NaCl (optimum, 0–1 %),
but not in the presence of 4% (w/v) NaCl. Does not produce fluorescent pigment on
King’s B media.Facultatively anaerobic.
incubation at 30 ºC on TSA plates. Growth occurs at 10-37 ºC (optimum, 30 ºC), at pH
6.0-10.0 (optimum, pH 8.0) and in the presence of 0-3% (w/v) NaCl (optimum, 0–1 %),
but not in the presence of 4% (w/v) NaCl. Does not produce fluorescent pigment on
King’s B media.Facultatively anaerobic.
Isolated from the sludge of an anaerobic, denitrifying, sulfide-removal bioreactor.
Undetermined.
- Tan WB, Jiang Z, Chen C, Yuan Y, Gao LF, Wang HF, Cheng J, Li WJ, Wang AJ. Thiopseudomonas denitrificans gen. nov., sp. nov.,
isolated from anaerobic activated sludge. Int J Syst Evol Microbiol 2015; 65:225-229. - Drobish AM, Emery BD, Whitney AM, Lauer AC, Metcalfe MG, McQuiston JR. Oblitimonas alkaliphila gen. nov., sp. nov., in the family
Pseudomonadaceae, recovered from a historical collection of previously unidentified clinical strains. Int J Syst Evol Microbiol
2016; 66:3063-3070.
(c) Costin Stoica
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