(c) Costin Stoica
Antibiogram optimization
Measured diameter of the inhibition zones are compared with the standard values
and sorted in descending order of antibacterial activity (also bacterial strain is
marked as 'susceptible', 'intermediate' or 'resistant' accordingly to the used
antibiotics).
Software database contains CLSI and EUCAST recommended antibiotics for
Enterobacteriaceae, Pasteurellaceae, Enterococcus, Neisseria, Staphylococcus,
Streptococcus and Pseudomonas.
The automatic inhibition zone diameter measurement function based on user-
uploaded images was recently added.
The history of ABIS
The project started in 2003 as a MS-DOS-based program for clinically significant
Enterobacteriaceae identification, using biochemical characters, morphology,
growth conditions, ecology and pathogenicity data.
In one year program reached version 4, upgrading with 3 more genera: Bacillus,
Streptococcus and Staphylococcus.
ABIS 5 was released in 2005 with major changes including an easy to use
Windows based interface, step by step identification for Gram-positive and
Gram-negative bacteria, rods and cocci (genera only), identification maps and 2
more families included: Pasteurellaceae (Pasteurella, Actinobacillus,
Haemophilus) and Pseudomonadaceae.
Also, Bacillus, Enterobacteriaceae and Staphylococcus databases were
updated and the biochemical tests list, used for identification, was extended.
Continuous changes in bacterial taxonomy forced us to stop the development of
the stand-alone version and focus on the online development. In the year 2007
"ABIS 6 online", linked with a germs Encyclopedia and hosted by 'Regnum
Prokaryotae" site, was officially released for public use. Later, "ABIS online" got
Vibironaceae identification (2010), family Campylobacteraceae (2012) genus
Clostridium and Salmonella Kauffman-White scheme (2013). Other upgrades
followed up to actual ABIS: Helicobacter and Lactobacillus (2014), Listeria and
Erysipelothrix (2014), Corynebacterium and Lancefield grouping for streptococci
(2015), Neisseria (2016), Chromobacterium (2018), Mycobacterium (2020) and
Bifidobacterium (2022).
Since then, the ABIS databases have continued to be periodically updated with
new species and genera; the development log can be seen in detail [here]
Additional functions recently added based on user-uploaded images: Automatic
inhibition zone diameter measurement and colony counting.


ABIS online Bacteria identification
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ATI 1.0 Antibiogram analyser
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ABIS online
ABIS online - Advanced Bacterial Identification Software is a laboratory tool for
bacterial identification, based on morpho-biochemical characters, cultural
characteristics, growth conditions, ecology and pathogenicity data.
The program allows a great flexibility in choosing biochemical tests and is an
alternative to commercial systems or code-books. The software is linked with a
bacterial Encyclopedia, the Kauffman-White scheme for Salmonella serovars
identification, streptococci Lancefield grouping, and an antibiogram interpreter
(diffusimetric method).
Main identifiable taxa: Enterobacteriaceae, Pasteurellaceae, Campylobacteraceae,
Bacillaceae, Lactobacillus, Staphylococcus, Streptococcus, Clostridium, Vibrio,
Listeria, Erysipelothrix, Mycobacterium, Corynebacterium, Neisseria,
Chromobacterium, Aeromonas, Pseudomonas and related species.
The number of taxa included in database is constantly expanding, and now, the
database had exceeded 3200 species.
Download Android Application
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The APP can be downloaded and installed as free application, outside the Google
play store. If your mobile device does not allow installing from 3rd party sources,
then follow the steps below:
1. Go to "Settings",
2. Go to "Security" or "Lock screen and security",
3. Scroll down and check “Unknown sources” box,
4. Tap ‘OK” when the warning pops up,
5. Download APK and install it.
For Android 8.0 or later go to “Settings => Apps & notifications => Advanced =>
Special app access => Install unknown apps.” Here, select the app (usually your
Internet browser) that you want to grant permission to install from unknown
sources. Then once you’ve tapped it, tap “Allow from this source” to enable
unknown sources for that specific app.
MS-DOS-based ABIS in 2003
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MS-DOS-based antibiogram interpreter
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Windows-based ABIS in 2005
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Selecting antibiogram database
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